Plant Extract Manufacturing Three Ways to Identify?

Plant Extract Manufacturing Three Ways to Identify?

What are the common plant extract manufacturing detection methods in the plant extraction industry? Today we will bring you HPLC detection methods. Let’s see what is HPLC? What is the HPLC process?

What is HPLC?

HPLC stands for High Performance Liquid Chromatography. It also known as “high pressure liquid chromatography,” “high speed liquid chromatography,” “high resolution liquid chromatography,” “modern column chromatography” and so on.

High performance liquid chromatography (HPLC) is an analytical method in the late 1960s. In recent years, it widely uses in the separation and determination of functional components of health food, nutritional fortifiers, vitamins and proteins. About 80% of organic compounds in the world can be analyzed by HPLC.

Characteristics of HPLC

High performance liquid chromatography (HPLC) has the characteristics of high pressure, high efficiency, high speed, high sensitivity and high selectivity. It widely uses in pharmaceutical, environmental protection, petrochemical, food, chemical and other industries. And is now an important method for separation and analysis.

Flow Chart for High Performance Liquid Chromatography

The solvent in the liquid storage bottle is sucked into the chromatography system by the pump. And then output, and after the flow and pressure measurement, it imports into the sampler. The detected object injects into the sample injector and passes through the column with the mobile phase. After separation on the column, the detected signal collects and processes by the data processing equipment, and the chromatogram records. Waste liquid flows into the waste liquid bottle. The gradient controller can also use for gradient elution in the case of complex mixture separation. This is similar to the programmed temperature of gas chromatography, except that gas chromatography changes the temperature. While HPLC changes the polarity of the mobile phase, so that the sample components can separate under the best conditions.

The separation process of high performance liquid chromatography

Like other chromatographic processes, HPLC is a continuous multi-exchange process between the stationary phase and the mobile phase. It separates different solutes by the difference of partition coefficient, affinity, adsorption force or molecular size between two phases.

Start the sample by adding it to the stigma. Assume that the sample contains 3 components, A, B and C, which enter the column along with the mobile phase and begin to be divided between the stationary phase and the mobile phase. The component A with small partition coefficient is not easily blocked by the stationary phase and flows out of the column earlier. The component C with a large partition coefficient stayed on the stationary phase for a long time and left the column later. The partition coefficient of component B is between A and C, and the second flows out of the column. If a mixture containing multiple components enters the system, each component in the mixture flows out of the column. According to its partition coefficient between the two phases in different order to achieve the purpose of separation.

The separation of different components in the chromatographic process depends first on whether there are differences in the partition coefficient, adsorption capacity and affinity of each component between the two phases, which is a thermodynamic equilibrium problem and the primary condition for separation. Secondly, when the different components move in the column, the spectral band is broadened with the column length, and the separation is related to the diffusion coefficient between the two phases, the size of the stationary phase, the packing of the column and the flow rate of the mobile phase. Therefore, the final effect of separation is the comprehensive benefit of both thermodynamics and kinetics.

hjtpharm plant extract manufacturing
Application areas of plant extract manufacturing process

Application areas of plant extract manufacturing process

Plant extract manufacturing process is from the plant as raw materials. Through physical and chemical extraction separation to obtain some or more active ingredients in the plant.

Although plant extract manufacturing process come in many forms, they share a common feature. The extracts represent naturally occurring phytochemicals that removes from the inert structure of the plant that produced them. The main advantage of using extracts from plants is that phytochemicals are more easy to absorb.

hjtpharm plant extract manufacturing

Application areas of plant extracts:

  1. Food Stuffs

According to the different needs of different people and different physiological conditions, as well as the principle of nutritional diet and physiological function adjustment. The food formula with different nutritional and health care functions designs in a targeted manner. And a large number of experiments also conducts to confirm that these different formulations of food do have their own required functional properties.

  1. feed

The new variety feed has listed 117 kinds of natural plants that can be fed with the same food and medicine. In the catalogue of feed additives, 12 kinds of plant extracts such as Eucommia ulmoides leaf extract lists. These raw materials and additives use for animal intestinal health and immunity.

  1. Health Products

Used to improve memory, anti-fatigue; Stress; Regulating blood lipids; Gastrointestinal function; Sleep; Anaemia; Protection against chemical liver damage; Milk secretion; Visual fatigue; Promoting lead removal; Beauty; Hair care.

  1. API

At present, pharmaceutical active ingredients obtains from plant extracts: such as paclitaxel. Ginsenosides, etc.

  1. Daily Chemicals and Cosmetics

It can be used for moisturizing cosmetics, water and oil balance, skin problems, etc.

  1. Natural pigment

With roots, stems, leaves, flowers, fruits, etc. of widely cultivated plants as initial materials. The color organic matter extractes from appropriate solvent and prepared by separation, refining and drying process. This kind of organic matter calls natural pigment of plant extract. At present, there are more than 40 kinds of main natural pigments on the market. Such as capsicum red pigment, marigold extract, gardenia yellow pigment, curcumin and so on.

  1. Natural sweeteners

Natural sweeteners are commonly referred to as natural sweeteners when the sweeteners are extracted and processed from natural plants. According to the sweetness, it can be divided into low-fold sweeteners such as sucrose, beet sugar. And high-power sweeteners such as stevioside, grosvenor momordica fruit sweetener, rubiside, liquorice extract, neohesperidin dihydrochalcone and the like. The gradual replacement of sugar with natural high-power sweeteners is currently the main trend in the food and beverage industry.

What is montmorillonite?

What is montmorillonite?

Montmorillonite is also called microcrystalline kaolinite. It is a layered structure, flake crystalline silicate clay ore. Named after its first discovery in the city of Monte, France. Its name is Guanyintu, and there are 11 species of its family.

Montmorillonite belongs to the Smectite family, the dioctahedron subfamily, A 2: 1 crystal water containing structure is formed by two layers of silicon-oxygen tetrahedron connected with a layer of aluminum (magnesium) oxygen (hydroxide) octahedra connected with common edges. It is one of the most varied crystal structures in clay minerals. The experimental results of slow scanning by diffractometer show that it is natural nano-materials.

Montmorillonite has the function of ion exchange. The first is the cation such as Na +, Ca2 +, K +, Mg2 ~ +, Li +, H. +, Al3 +, etc. The exchange is reversible. In the medium with pH 7, the cation exchange capacity is 70 ~ 140 me / 100 g.

Montmorillonite usually contains three conditions of water. Surface free of water, interlayer adsorption of water and lattice water. It has strong water absorption and swells after water absorption, that is, the distance between the lattice bottom surface increases. The crystal axis C0 can reach 1.84 ~ 2.14 nm at high hydration state. In aqueous media it can be dispersed in colloidal state. It is quickly lost in water when heated. 100 ~ 200 °C off the adsorbed water, 500 °C when a lot of lattice water began to escape.

Montmorillonite is electronegative. It has great influence on colloid property and rheological function. Electronegativity mainly comes from three aspects:

  1. The lattice displacement together with the internal compensating displacement forms the lattice electrostatic charge. Each unit cell is about 0.66 nm;
  2. Electronegativity of broken bonds;
  3. Electronegativity formed by dissociation of octahedral sheets.

Now there are two definitions of montmorillonite products:

  1. An explanation of the montmorillonite products of the metal mine industry: The content of smectite in the clay mine is greater than 80%. Such as the desiccant, etc. It is a primary component of bentonite. However, bentonite is not montmorillonite, and smectite is not bentonite. It only needs to be purified from bentonite.
  2. The other is the requirements of medical cosmetics and other professions for its products. This is the true meaning of montmorillonite. Its concept is close to the definition of montmorillonite in the field of scientific research. Its product content is qualitative and quantitative by XRD and other methods. The animal montmorillonite described in this paper is the product of this layer.

Montmorillonite is used in animal breeding, and the product must be purified. It is necessary to determine non-toxic (arsenic, mercury, lead, quartzite does not overspend). Any direct use of bentonite raw ore for animal breeding will cause damage to livestock and secondary damage to people.

Hongjitang Montmorillonite
Uses and Benefits of Plant Extracts

Uses and Benefits of Plant Extracts

The value of plants and plant remove has grown enormously during the current years. Since endless time plants have played a major duty in securing the atmosphere. Removes from plants have actually known to possess numerous useful high qualities to augment the total wellness of humans.

There more than a million types of essences derived from plants which can be made use of for several objectives. Researches have actually confirmed that plant extract can be used to cure numerous health and wellness disorders and it has lower side effects contrasted to various other forms of medicines.

Various Types of Plant extract from Plants and Their Benefits

Castor Oil:

Castor oil is a plant extract obtained from the castor seed. It has innumerable top qualities and is made use of for treating skin conditions.

Turmeric extract:

Turmeric is an additional plant essence which has a variety of medical virtues. Turmeric is taken into consideration a necessary source of antibiotics. It is made use of as a disinfectant in case of cuts as well as burns. This certain plant remove is additionally made use of by doctors to cure cancers. Certain sorts of cancers cells can be avoided by the intake of turmeric extract. It likewise aids to stop chronic heart ailments. Turmeric extract is even used on the face and also neck to prevent growth of hair. It likewise aids to maintain the skin clear as well as avoids acne and also acnes.

Aloe Vera:

This exemplary plant extract is taken into consideration special with amazing health advantages. Aloe vera is utilized for many purposes like treating skin diseases as it consists of vital oils and also minerals which are beneficial to the skin. It is also known to treat gall bladder problems, cancers cells as well as strengthen the body immune system of the body. Aloe Vera is made use of as an ingredient in many cosmetic items like hair shampoos as well as lotions. It is also used to treat first degree burns.


Known for its wonderful odor, this plant essence has a variety of valuable high qualities. It is known to cure cuts as well as attacks. Lavender is also considered a reliable medication to treat skin diseases like dermatitis and also psoriasis. Made use of as a moderate sedative, it also helps to maintain the skin oil cost-free.

It is considered an efficient antibacterial as well as used to cure many diseases. Neem essence is also considered an effectual cure for acne and boils.

Contrasted to various other kinds of medicines, the Plant extractoriginated from herbs and plants are much effective and also cheaper. As increasingly more people opt for medicines from plant extract, purchasers discover it easy to acquire these products through on the internet markets too.

With a number of healing homes, medications originated from plant essence are widely used by many people throughout the world. In addition to curing skin diseases as well as persistent ailments, it is also utilized to restore hormonal discrepancies and other diseases.

The relevance of plants and also plant remove has actually expanded greatly throughout the current years. Plant extractfrom plants have actually recognized to have several helpful qualities to increase the overall wellness of human beings.

Castor Oil:

Castor oil is a plant extract derived from the castor seed. Aloe Vera: This excellent plant remove is thought about distinct with amazing health benefits. Lavender: Known for its pleasant smell, this plant essence has a number of valuable qualities.



Basic concept

Allulose 3-epimerase (CCDPEase) is fused with sesame oil body protein as a six-carbon rare ketose with almost zero calories, and insoluble protein in E. coli After heterologous expression, the inclusion body protein was further mixed with triglycerides and phospholipids to construct artificial oil bodies (Artificial Oil Bodies, AOBs). In AOBs, the lipophilic part of Oleosin lipoprotein is embedded in the triglyceride core region, and the CCDPEase enzyme protein is fused with the AOBs arm composed of amphiphilic molecules and displayed on the surface of AOBs. its biotransformation function

Physiological function

Effects of Alluloseon lipid metabolism

Hossain et al. added 5% allulose to the drinking water of spontaneously type 2 diabetic OLETF (Otsuka Long-Evans Tokushima Fatty) rats, and found that the abdominal fat and body fat weight of the rats were significantly lower than those of the control group after feeding for 13 weeks. And fat cells were smaller than the control group. When different doses of Allulosewere added to the normal diet of high-fat diet-induced obese rats, it was found that the body weight and the amount of body fat accumulated were decreased compared with the control group, and the degree of reduction was significantly different with psicose. dose-dependent relationship. Rats were fed 3% psicose for 4 weeks, and it was found that the Allulosegroup had significantly lower serum insulin and leptin levels, decreased liver liposynthase activity, and lipoxygenase expression levels It is speculated that Allulosecan reduce fat accumulation by inhibiting fat synthesis and increasing the rate of lipolysis.

Effects of Alluloseon blood glucose metabolism

Several studies have shown that Allulosehas a hypoglycemic effect. Male rats were fed sucrose, maltose or soluble starch, and at the same time added Alluloseor D-fructose by 1/1000, it was found that psicose could inhibit the concentration of glucose in plasma; in rat experiments, it was found that, After oral administration, allulose will be absorbed into the blood through the small intestine, and then excreted by the kidneys, which will not cause blood sugar fluctuations, and can also inhibit the activity of α-glucosidase.

Anti-diabetic mechanism of D-psicose

The OLETF rat experiment carried out by Hossain et al. showed that postprandial blood glucose, body weight and fat in the psicose group were effectively controlled. The immunohistochemical results showed that psicose could induce the expression of hepatic glucokinase, thereby increasing the synthesis of hepatic glycogen. . Further research found that allulose can slow down the fibrosis of beta islet cells. The experimental time was extended to 60 weeks, and it was found that the anti-type 2 diabetes effect of Allulosewas mainly achieved by maintaining blood sugar levels, reducing weight gain, controlling postprandial blood sugar, reducing inflammation, and reducing glycated hemoglobin levels.